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Bassik Human CRISPR Knockout Library
(Pooled Libraries #101926, #101927, #101928, #101929, #101930, #101931, #101932, #101933, #101934)

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  • Purpose

    This lentiviral genome-scale CRISPR library targets all ~20,500 protein-coding genes in the human genome. The library is divided into 9 sublibraries defined by biological categories of genes, each with their own negative controls.

    This library includes safe-targeting controls, gRNAs which target the genome but are not expected to disrupt gene function, in addition to nontargeting controls. The gRNAs vary in length from 17-20 bp, with the truncated gRNAs (17 or 18 bp) having less off-target activity.

  • Vector Backbone

    pMCB320 (Plasmid #89359) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 101926 Human CRISPR Deletion Library - Apoptosis and cancer† 1 $540 Add to Cart
Pooled Library 101927 Human CRISPR Deletion Library - Drug targets, kinases, phosphatases† 1 $540 Add to Cart
Pooled Library 101928 Human CRISPR Deletion Library - Gene expression† 1 $540 Add to Cart
Pooled Library 101929 Human CRISPR Deletion Library - Membrane proteins† 1 $540 Add to Cart
Pooled Library 101930 Human CRISPR Deletion Library - Proteostasis† 1 $540 Add to Cart
Pooled Library 101931 Human CRISPR Deletion Library - Trafficking, mitochondrial, motility† 1 $540 Add to Cart
Pooled Library 101932 Human CRISPR Deletion Library - Unassigned 1† 1 $540 Add to Cart
Pooled Library 101933 Human CRISPR Deletion Library - Unassigned 2† 1 $540 Add to Cart
Pooled Library 101934 Human CRISPR Deletion Library - Unassigned 3† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with plasmids expressing Cas9 such as lentiCas9-Blast (#52962) or otherwise used with cell lines already expressing Cas9.

  • If you would like to receive all nine sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted gRNAs
101926 3015 31,324
101927 2333 24,569
101928 2345 24,449
101929 1098 12,334
101930 2927 30,423
101931 2252 23,615
101932 1954 20,482
101933 2771 28,187
101934 1854 17,438
  • Controls
    ~5600 unique non-targeting (750 per sublibrary - partial overlap between sublibraries); 6750 unique safe-targeting (750 per sublibrary)
  • Species
    Human
  • Lentiviral Generation
    3rd

    Library Shipping

    Each library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

    • Volume
      ∼10µL
    • Concentration
      10ng/µL

Resource Information

Depositor Comments

The bioinformatic pipeline for analysis is available at bitbucket.org/dmorgens/castle and is described in Morgens et al. Nature Biotechnology 2016.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human CRISPR Deletion Library - Apoptosis and cancer was a gift from Michael Bassik (Addgene #101926)
    Human CRISPR Deletion Library - Drug targets, kinases, phosphatases was a gift from Michael Bassik (Addgene #101927)
    Human CRISPR Deletion Library - Gene expression was a gift from Michael Bassik (Addgene #101928)
    Human CRISPR Deletion Library - Membrane proteins was a gift from Michael Bassik (Addgene #101929)
    Human CRISPR Deletion Library - Proteostasis was a gift from Michael Bassik (Addgene #101930)
    Human CRISPR Deletion Library - Trafficking, mitochondrial, motility was a gift from Michael Bassik (Addgene #101931)
    Human CRISPR Deletion Library - Unassigned 1 was a gift from Michael Bassik (Addgene #101932)
    Human CRISPR Deletion Library - Unassigned 2 was a gift from Michael Bassik (Addgene #101933)
    Human CRISPR Deletion Library - Unassigned 3 was a gift from Michael Bassik (Addgene #101934)

  • For your References section:

    Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens. Morgens DW, Wainberg M, Boyle EA, Ursu O, Araya CL, Tsui CK, Haney MS, Hess GT, Han K, Jeng EE, Li A, Snyder MP, Greenleaf WJ, Kundaje A, Bassik MC. Nat Commun. 2017 May 5;8:15178. doi: 10.1038/ncomms15178. PubMed 28474669
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