WPXL LEFTY2 gRNA
(Plasmid
#101924)
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PurposeLEFTY2 gRNA used for targeted demethylation is inserted into the WPXL lentiviral backbone.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101924 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneWPLX destination vector
- Backbone size w/o insert (bp) 12806
- Total vector size (bp) 11630
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Modifications to backboneremoved Puromycin selection and inserted Hygromycin
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Vector typeLentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLEFTY2 enhancer targeting gRNA
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gRNA/shRNA sequenceGAGAAGCAGAGCAGGAAGTC
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SpeciesH. sapiens (human)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer TCTCGACGGTATCGGTTAACTT
- 3′ sequencing primer TACGGGAAGCAATAGCATGA (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
WPXL LEFTY2 gRNA was a gift from Danwei Huangfu (Addgene plasmid # 101924 ; http://n2t.net/addgene:101924 ; RRID:Addgene_101924) -
For your References section:
TET proteins safeguard bivalent promoters from de novo methylation in human embryonic stem cells. Verma N, Pan H, Dore LC, Shukla A, Li QV, Pelham-Webb B, Teijeiro V, Gonzalez F, Krivtsov A, Chang CJ, Papapetrou EP, He C, Elemento O, Huangfu D. Nat Genet. 2018 Jan;50(1):83-95. doi: 10.1038/s41588-017-0002-y. Epub 2017 Dec 4. 10.1038/s41588-017-0002-y PubMed 29203910
Map uploaded by the depositor.
