pEGFP Sec22b
(Plasmid #101918)

• Purpose: Expresses Sec22b in mammalian cells
• Depositing Lab: Thierry Galli
• Publication: Petkovic et al Nat Cell Biol. 2014 May;16(5):434-44. doi: 10.1038/ncb2937. Epub 2014 Apr 6.

Backbone

• Vector backbone: pEGFP C1
• Backbone manufacturer: CLONETECH
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5333
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Sec22b
• Alt name: SEC22L1
• Alt name: ERS-24
• Alt name: SEC22 Vesicle Trafficking Protein Homolog B
• Species: M. musculus (mouse)
• Insert Size (bp): 633
• GenBank ID: 9554
• Entrez Gene: Sec22b (a.k.a. 4930564D15Rik, AA517334, AI480645, C81333, ERS-24, Sec22l1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP C
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry