pEGFP SNAP47
(Plasmid #101915)

• Purpose: Expresses SNAP47 in mammalian cells
• Depositing Lab: Thierry Galli
• Publication: Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-69. doi: 10.1074/jbc.M115.666362. Epub 2015 Sep 10.

Backbone

• Vector backbone: pEGFP C3
• Backbone size w/o insert (bp): 1341
• Total vector size (bp): 6041
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SNAP47
• Alt name: synaptosome associated protein 47, C1orf142, HEL170, SVAP1
• Species: H. sapiens (human)
• Insert Size (bp): 1341
• GenBank ID: 116841
• Entrez Gene: SNAP47 (a.k.a. C1orf142, ESFI5812, HEL-S-290, HEL170, SNAP-47, SVAP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: EGFP C
• 3′ sequencing primer: EGFP-N

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Proux-Gillardeaux V., Galli T
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry