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PurposeExpresses SNAP23 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101914 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP C3
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Backbone manufacturerCLONETECH
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5336
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSNAP 23
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Alt namesynaptosome associated protein 23
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SpeciesH. sapiens (human)
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Insert Size (bp)636
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GenBank ID8773
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Entrez GeneSNAP23 (a.k.a. HsT17016, SNAP-23, SNAP23A, SNAP23B)
- Promoter CMV
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer EGFP-C
- 3′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP SNAP23 was a gift from Thierry Galli (Addgene plasmid # 101914 ; http://n2t.net/addgene:101914 ; RRID:Addgene_101914) -
For your References section:
The Q-soluble N-Ethylmaleimide-sensitive Factor Attachment Protein Receptor (Q-SNARE) SNAP-47 Regulates Trafficking of Selected Vesicle-associated Membrane Proteins (VAMPs). Kuster A, Nola S, Dingli F, Vacca B, Gauchy C, Beaujouan JC, Nunez M, Moncion T, Loew D, Formstecher E, Galli T, Proux-Gillardeaux V. J Biol Chem. 2015 Nov 20;290(47):28056-69. doi: 10.1074/jbc.M115.666362. Epub 2015 Sep 10. 10.1074/jbc.M115.666362 PubMed 26359495