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Addgene

5TEY (METTL3)
(Plasmid #101892)

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Item Catalog # Description Quantity Price (USD)
Plasmid 101892 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pFBOH-MHL
  • Backbone manufacturer
    Cheryl Arrowsmith (Addgene plasmid # 62304)
  • Vector type
    Baculovirus expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    METTL3
  • Alt name
    gi|99077115
  • Species
    H. sapiens (human)
  • Entrez Gene
    METTL3 (a.k.a. IME4, M6A, MT-A70, Spo8, hMETTL3)
  • Promoter polyhedrin
  • Tag / Fusion Protein
    • 6xHis, TEV (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
  • 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3')
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: METTL3:JMC094-C09:C231635. SGC or PDG link: http://www.thesgc.org/structures/5TEY/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    5TEY (METTL3) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101892 ; http://n2t.net/addgene:101892 ; RRID:Addgene_101892)
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