5TEY (METTL14)
(Plasmid
#101891)
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PurposeBaculovirus expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101891 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * |
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Backbone
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Vector backbonepFBOH-LIC notag
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Vector typeBaculovirus expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMETTL14
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Alt namegi|34147341
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SpeciesH. sapiens (human)
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Entrez GeneMETTL14 (a.k.a. hMETTL14)
- Promoter polyhedrin
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pFBOH-fwd (5'-CCGGATTATTCATACCGTCCCACCA-3')
- 3′ sequencing primer pFBOH-rev (5'-CTGATTATGATCCTCTAGTACTTCT-3') (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
N terminal tag: none. SGC Clone Sample ID: METTL14:JMC097-G06:C232534. SGC or PDG link: http://www.thesgc.org/structures/5TEY/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5TEY (METTL14) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101891 ; http://n2t.net/addgene:101891 ; RRID:Addgene_101891)