5KCH (SETDB1)
(Plasmid #101881)

• Purpose: Bacterial expression for structure determination; may not be full ORF
• Depositing Lab: Cheryl Arrowsmith
• Publication: SGC Toronto Q2 Q3 2016 (unpublished)

Backbone

• Vector backbone: pET28-MHL
• Backbone manufacturer: Cheryl Arrowsmith (Addgene plasmid # 26096)
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SETDB1
• Alt name: gi|41281392
• Alt name: PDB IDs: 5KCH, 6AU2, 6AU3, 6BPI, 5KCO, 5KE2, 5KE3, 5KH6
• Alt name: BC028671
• Species: H. sapiens (human)
• Entrez Gene: SETDB1 (a.k.a. ESET, H3-K9-HMTase4, KG1T, KMT1E, TDRD21)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis, TEV (N terminal on backbone)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

N terminal tag: MHHHHHHSSGRENLYFQG. SGC Clone Sample ID: SETDB1:APC043-C07:C27642.

SGC or PDG links:
5KCH http://www.thesgc.org/structures/5KCH/
6AU2 http://www.thesgc.org/structures/6AU2/
6AU3 http://www.thesgc.org/structures/6AU3/
6BPI http://www.thesgc.org/structures/6BPI/
5KCO http://www.thesgc.org/structures/5KCO/
5KE2 http://www.thesgc.org/structures/5KE2/
5KE3 http://www.thesgc.org/structures/5KE3/
5KH6 http://www.thesgc.org/structures/5KH6/