5KO9 (HMCES)
(Plasmid
#101877)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101877 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepNIC-CH
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Backbone manufacturerOpher Gileadi (Addgene plasmid # 26117)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHMCES
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Alt namePDB ID: 5K09, 6OE7, 6OEA, 6OEB
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SpeciesH. sapiens (human)
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Entrez GeneHMCES (a.k.a. C3orf37, DC12, SRAPD1)
- Promoter T7
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Tag
/ Fusion Protein
- 6x His (modified C-term tag is AENLYFQSHHHHHH) (C terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
C terminal tag: AENLYFQSHHHHHH. SGC Clone Sample ID: HMCES:PBC006-B11:C236394. SGC or PDG link:
http://www.thesgc.org/structures/5KO9/
https://www.thesgc.org/structures/6OE7/
https://www.thesgc.org/structures/6OEA/
https://www.thesgc.org/structures/6OEB/
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
5KO9 (HMCES) was a gift from Cheryl Arrowsmith (Addgene plasmid # 101877 ; http://n2t.net/addgene:101877 ; RRID:Addgene_101877)
