pEYFP-N1-Pink1
(Plasmid #101874)

• Purpose: Transient expression of Pink1-YFP in mammalian cells
• Depositing Lab: Richard Youle
• Publication: Narendra et al PLoS Biol. 2010 Jan 26;8(1):e1000298. doi: 10.1371/journal.pbio.1000298.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 6452
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTEN induced putative kinase 1
• Alt name: PTEN induced putative kinase 1
• Species: H. sapiens (human)
• Insert Size (bp): 1745
• GenBank ID: NM_032409.2
• Entrez Gene: PINK1 (a.k.a. BRPK, PARK6)
• Promoter: CMV
• Tag / Fusion Protein:
  • fluorescent tag EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GTC GTA ACA ACT CCG CCC
• 3′ sequencing primer: GTC CAG CTC GAC CAG GAT G

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry