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Purpose(Empty Backbone) Lentiviral empty backbone, dual BFP and puro markers
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101864 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLV-EF1a-PGK-puro
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Backbone manufacturerProvided by N. Hathaway (as N103), Crabtree lab
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Vector typeMammalian Expression, Lentiviral
- Promoter EF1a
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This is a modified version of pLV-EF1a-PGK-Puro. The puro-T2a-BFP marker was inserted from Addgene plasmid #50946. T2a is a linker, causing one RNA to be produced, resulting in two (BFP and puro) polypeptides.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKAM-BFP-Puro was a gift from Archibald Perkins (Addgene plasmid # 101864 ; http://n2t.net/addgene:101864 ; RRID:Addgene_101864)
Map uploaded by the depositor.
