lentiCRISPRv2-TTF-1-gRNA
(Plasmid #101857)

• Purpose: Introducing a gRNA targeting human TTF-1 (NKX2-1) into cells
• Depositing Lab: David Mu
• Publication: Lai et al Oncogene. 2018 Mar 19. pii: 10.1038/s41388-018-0174-7. doi: 10.1038/s41388-018-0174-7.

Backbone

• Vector backbone: lentiCRISPRv2
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: gRNA targeting TTF-1
• gRNA/shRNA sequence: GGGGCTCCGCTGGCGGCGTAC
• Species: H. sapiens (human)
• GenBank ID: NM_003317
• Entrez Gene: NKX2-1 (a.k.a. BCH, BHC, NK-2, NKX2.1, NKX2A, NMTC1, T/EBP, TEBP, TITF1, TTF-1, TTF1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsmBI (unknown if destroyed)
• 3′ cloning site: BsmBI (unknown if destroyed)
• 5′ sequencing primer: GAGGGCCTATTTCCCATGATT
• 3′ sequencing primer: CATAGCGTAAAAGGAGCAACA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry