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Addgene

P0050::kaiBC S431A T432A NS1
(Plasmid #101837)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101837 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAM1303
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 8100
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin and Streptomycin, 50 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    kaiBC S431A T432A with p0050 promoter
  • Species
    S. elongatus PCC7942
  • Insert Size (bp)
    2400
  • Mutation
    S431A T432A

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P0050::kaiBC S431A T432A NS1 was a gift from Erin O'Shea (Addgene plasmid # 101837 ; http://n2t.net/addgene:101837 ; RRID:Addgene_101837)

  • For your References section:

    Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway. Puszynska AM, O'Shea EK. Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210. 10.7554/eLife.23210 PubMed 28430105
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