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Addgene

P0050::rpaAD53A
(Plasmid #101831)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101831 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 7100
  • Vector type
    Cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RpaA D53A with p0050 promoter (+ gentamicin resistance casette)
  • Species
    S. elongatus PCC7942
  • Insert Size (bp)
    3100
  • Mutation
    D53 mutated to A
  • GenBank ID
    WP_011243721.1

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    P0050::rpaAD53A was a gift from Erin O'Shea (Addgene plasmid # 101831 ; http://n2t.net/addgene:101831 ; RRID:Addgene_101831)

  • For your References section:

    Switching of metabolic programs in response to light availability is an essential function of the cyanobacterial circadian output pathway. Puszynska AM, O'Shea EK. Elife. 2017 Apr 21;6. pii: e23210. doi: 10.7554/eLife.23210. 10.7554/eLife.23210 PubMed 28430105
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