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Addgene

pCRRNA-G10-R20
(Plasmid #101819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101819 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCRRNAcos
  • Backbone size w/o insert (bp) 2870
  • Vector type
    CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crRNAs targeting GFP and mCherry
  • gRNA/shRNA sequence
    sfGFP crRNA: CCTTCACCTTGTGGTGCTTGAGAGAATTTG; mCherry crRNA: AGTTTGGCGGTCTGGGTGCCTTCATACGGA
  • Species
    Synthetic
  • Promoter Spy 1049

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer TTACTTTGCAGGGCTTCCCAACC
  • 3′ sequencing primer TTTGATGCCTCTAGCACGCG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCRRNA-G10-R20 was a gift from David Bikard & Sven van Teeffelen (Addgene plasmid # 101819 ; http://n2t.net/addgene:101819 ; RRID:Addgene_101819)

  • For your References section:

    Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes. Vigouroux A, Oldewurtel E, Cui L, Bikard D, van Teeffelen S. Mol Syst Biol. 2018 Mar 8;14(3):e7899. PubMed 29519933
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