pET28a-R4-uAb
(Plasmid #101801)

• Purpose: Bacterial cell expression of the beta-galactosidase-specific ubiquibody, R4-uAb
• Depositing Lab: Matthew DeLisa
• Publication: Portnoff et al J Biol Chem. 2014 Mar 14;289(11):7844-55. doi: 10.1074/jbc.M113.544825. Epub 2014 Jan 28.

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6616
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R4-uAb
• Alt name: scFv13-R4-GSGSG-CHIPΔTPR-FH
• Species: H. sapiens (human); Fusion Protein
• Insert Size (bp): 1365
• Promoter: T7
• Tags / Fusion Proteins:
  • Flag (C terminal on insert)
  • 6xHis (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Resource Information

• Supplemental Documents:
  • pET28a scFvR4-GSGSG-hCH flag HIS.gbk
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry