pCDF1-mAtf4-HT-puro
(Plasmid #101795)

• Purpose: Lentiviral vector containing C-terminally Halo-tag tagged mouse ATF4
• Depositing Lab: Seiichi Oyadomari
• Publication: Oyadomari Lab Luciferase reporters (unpublished)

Backbone

• Vector backbone: pCDF1-MCS2-EF1-Puro
• Backbone manufacturer: Systems Biosciences Inc
• Backbone size w/o insert (bp): 6592
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Activating transcription factor 4
• Alt name: Atf4
• Species: M. musculus (mouse)
• Insert Size (bp): 2004
• Entrez Gene: Atf4 (a.k.a. Atf-4, C/ATF, CREB-2, CREB2, TAXREB67)
• Tag / Fusion Protein:
  • Halo tag (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV Forward

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry