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PurposeRetroviral vector encoding C-terminally FLAG tagged mouse PERK
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMXs-IP
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Backbone manufacturerDr. Toshio Kitamura
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePerk
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Alt nameEif2ak3
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SpeciesM. musculus (mouse)
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Insert Size (bp)3559
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Entrez GeneEif2ak3 (a.k.a. Pek, Perk)
- Promoter LTR
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Tag
/ Fusion Protein
- 3xFLAG (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMXs-mPERK-FLAG-IP was a gift from Seiichi Oyadomari (Addgene plasmid # 101793 ; http://n2t.net/addgene:101793 ; RRID:Addgene_101793) -
For your References section:
Integrated stress response of vertebrates is regulated by four eIF2alpha kinases. Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S. Sci Rep. 2016 Sep 16;6:32886. doi: 10.1038/srep32886. 10.1038/srep32886 PubMed 27633668
