Skip to main content
Addgene

pMXs-mHRI-FLAG-IP
(Plasmid #101791)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101791 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pMXs-IP
  • Backbone manufacturer
    Dr. Toshio Kitamura
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Hri
  • Alt name
    Eif2ak1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1982
  • Entrez Gene
    Eif2ak1 (a.k.a. HCR, Hri)
  • Promoter LTR
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-mHRI-FLAG-IP was a gift from Seiichi Oyadomari (Addgene plasmid # 101791 ; http://n2t.net/addgene:101791 ; RRID:Addgene_101791)

  • For your References section:

    Integrated stress response of vertebrates is regulated by four eIF2alpha kinases. Taniuchi S, Miyake M, Tsugawa K, Oyadomari M, Oyadomari S. Sci Rep. 2016 Sep 16;6:32886. doi: 10.1038/srep32886. 10.1038/srep32886 PubMed 27633668
Related items:
Commonly requested with: