pMH-SFB-DYRK1B
(Plasmid
#101771)
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PurposeMammalian expression of DYRK1B fusion protein
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101771 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-SFB
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDYRK1B / MIRK
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SpeciesH. sapiens (human)
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MutationsiRNA-resistant allele
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Entrez GeneDYRK1B (a.k.a. AOMS3, MIRK)
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Tag
/ Fusion Protein
- S peptide-flag-streptavidin binding peptide (SFB) (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-SFB-DYRK1B was a gift from Michael Huen (Addgene plasmid # 101771 ; http://n2t.net/addgene:101771 ; RRID:Addgene_101771) -
For your References section:
Screen identifies DYRK1B network as mediator of transcription repression on damaged chromatin. Dong C, West KL, Tan XY, Li J, Ishibashi T, Yu CH, Sy SMH, Leung JWC, Huen MSY. Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17019-17030. doi: 10.1073/pnas.2002193117. Epub 2020 Jul 1. 10.1073/pnas.2002193117 PubMed 32611815