-
PurposeMammalian expression of DYRK1A fusion protein
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 101770 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepMH-SFB
-
Vector typeMammalian Expression
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameDYRK1A
-
SpeciesH. sapiens (human)
-
MutationsiRNA-resistant allele
-
Entrez GeneDYRK1A (a.k.a. DYRK, DYRK1, HP86, MNB, MNBH, MRD7)
-
Tag
/ Fusion Protein
- S peptide-flag-streptavidin binding peptide (SFB) (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMH-SFB-DYRK1A was a gift from Michael Huen (Addgene plasmid # 101770 ; http://n2t.net/addgene:101770 ; RRID:Addgene_101770) -
For your References section:
Nuclear F-actin assembly on damaged chromatin is regulated by DYRK1A and Spir1 phosphorylation. Li J, Xiong N, West KL, Leung M, Ching YP, Huang J, Yuan J, Yu CH, Leung J, Huen M. Nucleic Acids Res. 2024 Jul 5:gkae574. doi: 10.1093/nar/gkae574. 10.1093/nar/gkae574 PubMed 38966995