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Addgene

pMH-SFB-DYRK1A
(Plasmid #101770)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101770 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pMH-SFB
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DYRK1A
  • Species
    H. sapiens (human)
  • Mutation
    siRNA-resistant allele
  • Entrez Gene
    DYRK1A (a.k.a. DYRK, DYRK1, HP86, MNB, MNBH, MRD7)
  • Tag / Fusion Protein
    • S peptide-flag-streptavidin binding peptide (SFB) (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMH-SFB-DYRK1A was a gift from Michael Huen (Addgene plasmid # 101770 ; http://n2t.net/addgene:101770 ; RRID:Addgene_101770)
  • For your References section:

    Nuclear F-actin assembly on damaged chromatin is regulated by DYRK1A and Spir1 phosphorylation. Li J, Xiong N, West KL, Leung M, Ching YP, Huang J, Yuan J, Yu CH, Leung J, Huen M. Nucleic Acids Res. 2024 Jul 5:gkae574. doi: 10.1093/nar/gkae574. 10.1093/nar/gkae574 PubMed 38966995