TRAIP-SFB
(Plasmid
#101769)
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PurposeMammalian expression of TRAIP fusion protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101769 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneunknown
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTRAIP / RNF206
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SpeciesH. sapiens (human)
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MutationsiRNA-resistant allele
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Entrez GeneTRAIP (a.k.a. RNF206, SCKL9, TRIP)
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Tag
/ Fusion Protein
- S peptide-flag-streptavidin binding peptide (SFB) (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRAIP-SFB was a gift from Michael Huen (Addgene plasmid # 101769 ; http://n2t.net/addgene:101769 ; RRID:Addgene_101769) -
For your References section:
TRAIP regulates replication fork recovery and progression via PCNA. Feng W, Guo Y, Huang J, Deng Y, Zang J, Huen MS. Cell Discov. 2016 Jun 28;2:16016. doi: 10.1038/celldisc.2016.16. eCollection 2016. 10.1038/celldisc.2016.16 PubMed 27462463