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Purpose(Empty Backbone) Gateway-compatible destination vector for mammalian expression of N-terminal HA-tag fusion protein
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101767 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMH-HA
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Vector typeMammalian Expression
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMH-HA was a gift from Michael Huen (Addgene plasmid # 101767 ; http://n2t.net/addgene:101767 ; RRID:Addgene_101767) -
For your References section:
Dual-utility NLS drives RNF169-dependent DNA damage responses. An L, Jiang Y, Ng HH, Man EP, Chen J, Khoo US, Gong Q, Huen MS. Proc Natl Acad Sci U S A. 2017 Apr 4;114(14):E2872-E2881. doi: 10.1073/pnas.1616602114. Epub 2017 Mar 21. 10.1073/pnas.1616602114 PubMed 28325877
