CS2 Flag-Smad2 (3A)
(Plasmid #101763)

• Purpose: Expresses SMAD2 (3A) in mammalian cells
• Depositing Lab: Yuh-Shan Jou
• Publication: Yeh et al Nat Cell Biol. 2018 Apr;20(4):479-491. doi: 10.1038/s41556-018-0062-y. Epub 2018 Mar 28.

Backbone

• Vector backbone: pCS2
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SMAD2
• Alt name: SMAD family member 2
• Alt name: MADH
• Alt name: hMAD-2
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: Three Ser to Ala mutations in the phosphorylation sites (S464A, S465A, S467A)
• GenBank ID: NM_005901.5
• Entrez Gene: SMAD2 (a.k.a. CHTD8, JV18, JV18-1, LDS6, MADH2, MADR2, hMAD-2, hSMAD2)
• Promoter: CMV
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: EBV rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: This clone was modified from (Plasmid #14042) by three Ser to Ala mutations in the phosphorylation sites.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry