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Purpose(Empty Backbone) Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101750 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS305
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Modifications to backboneContains 2 micron ori and functional NatMX marker cassette.
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Vector typeYeast Expression
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Selectable markersNatMX
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRN1120 was a gift from Rene Verwaal (Addgene plasmid # 101750 ; http://n2t.net/addgene:101750 ; RRID:Addgene_101750) -
For your References section:
CRISPR/Cpf1 enables fast and simple genome editing of Saccharomyces cerevisiae. Verwaal R, Buiting-Wiessenhaan N, Dalhuijsen S, Roubos JA. Yeast. 2017 Sep 8. doi: 10.1002/yea.3278. 10.1002/yea.3278 PubMed 28886218
Map uploaded by the depositor.
