pCSN068
(Plasmid #101749)

• Purpose: Single copy yeast plasmid expressing Cpf1 from Francisella novicida U112 (FnCpf1), codon optimized for expression in Saccharomyces cerevisiae.
• Depositing Lab: Rene Verwaal
• Publication: Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.

Backbone

• Vector backbone: pRS414
• Total vector size (bp): 11538
• Vector type: Yeast Expression, CRISPR
• Selectable markers: TRP1 ; KanMX

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cpf1 from Francisella novicida U112 (FnCpf1) codon optimized for expression in S. cerevisiae.
• Alt name: FnCpf1
• Species: Francisella novicida U112
• Insert Size (bp): 3936
• Promoter: Heterologous promoter from K. lactis (KLLA0F20031g).
• Tag / Fusion Protein:
  • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: .
• 3′ sequencing primer: .

Gene/Insert 2

• Gene/Insert name: KanMX marker expression cassette.
• Promoter: Heterologous TEF1 promoter from A. gossypii.

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: .
• 3′ sequencing primer: .

Resource Information

• Supplemental Documents:
  • pCSN068.gb
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry