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pTT610
(Plasmid #101742)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101742 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTV118N
  • Backbone manufacturer
    Takara
  • Backbone size w/o insert (bp) 3095
  • Total vector size (bp) 7761
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Addition of 0.4% Glucose in the media.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thermus thermophilus HB8 SecY-A/SecE/SecG
  • Alt name
    SecYAEG
  • Species
    Thermus thermophilus HB8
  • Insert Size (bp)
    4666
  • Mutation
    SecY(L2V, R252G)–(GGSG)4–SecA1-939–His10 fusion protein, SecE and SecG
  • GenBank ID
    3169354 3169944
  • Entrez Gene
    secE (a.k.a. TTHA0249)
  • Entrez Gene
    secY (a.k.a. TTHA1672)
  • Promoter Lac
  • Tag / Fusion Protein
    • His10 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer AGCTCACTCATTAGGCAC
  • 3′ sequencing primer TCTTCGCTATTACGCCAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The SecA version used here is Cys-less, and the amino acid sequence contains the C648S variant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTT610 was a gift from Tomoya Tsukazaki (Addgene plasmid # 101742 ; http://n2t.net/addgene:101742 ; RRID:Addgene_101742)

  • For your References section:

    SecY-SecA fusion protein retains the ability to mediate protein transport. Sugano Y, Furukawa A, Nureki O, Tanaka Y, Tsukazaki T. PLoS One. 2017 Aug 18;12(8):e0183434. doi: 10.1371/journal.pone.0183434. eCollection 2017. PONE-D-17-20168 [pii] PubMed 28820900
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