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Purposeexpression of an optimized Cas9 for base-editing in zebrafish
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101740 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneCMV
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Backbone manufacturerorigene
- Backbone size w/o insert (bp) 3405
- Total vector size (bp) 8532
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namerAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
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SpeciesR. norvegicus (rat), Synthetic
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Insert Size (bp)5127
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MutationD10A, VQR mutations
- Promoter CMV
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer M13-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-BE-VQRCas9 was a gift from Shuo Lin (Addgene plasmid # 101740 ; http://n2t.net/addgene:101740 ; RRID:Addgene_101740) -
For your References section:
Programmable base editing of zebrafish genome using a modified CRISPR-Cas9 system. Zhang Y, Qin W, Lu X, Xu J, Huang H, Bai H, Li S, Lin S. Nat Commun. 2017 Jul 25;8(1):118. doi: 10.1038/s41467-017-00175-6. 10.1038/s41467-017-00175-6 [pii] PubMed 28740134
Map uploaded by the depositor.
