px459 EQR
(Plasmid
#101732)
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PurposeExpresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101732 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepx459 v2
- Total vector size (bp) 9175
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSpCas9 EQR
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SpeciesS pyogenes
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Insert Size (bp)4101
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MutationVQR
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cas9 EQR sequence cloned from Addgene Plasmid #65772 cloned into px459 Addgene plasmid #62988
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
px459 EQR was a gift from Andrew Holland (Addgene plasmid # 101732 ; http://n2t.net/addgene:101732 ; RRID:Addgene_101732) -
For your References section:
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. 10.1016/bs.mcb.2018.03.004 PubMed 29804665