Skip to main content
Addgene

p458 VRER
(Plasmid #101728)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101728 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    px458
  • Total vector size (bp) 9289
  • Vector type
    Mammalian Expression
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 VRER
  • Species
    S pyogenes
  • Insert Size (bp)
    4101
  • Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutations in Cas9)
  • Tags / Fusion Proteins
    • 3xFLAG-NLS (N terminal on insert)
    • NLS (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cas9 VRER sequence cloned from Addgene Plasmid #65773 cloned into px458 Addgene plasmid #48138

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p458 VRER was a gift from Andrew Holland (Addgene plasmid # 101728 ; http://n2t.net/addgene:101728 ; RRID:Addgene_101728)

  • For your References section:

    Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. 10.1016/bs.mcb.2018.03.004 PubMed 29804665
Related items:
Commonly requested with: