pCSN061
(Plasmid #101725)

• Purpose: Single copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.
• Depositing Lab: Rene Verwaal
• Publication: Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278.

Backbone

• Vector backbone: pRS414
• Vector type: Yeast Expression, CRISPR
• Selectable markers: TRP1 ; KanMX

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: S. pyogenes Cas9 codon optimized for expression in S. cerevisiae.
• Alt name: SpCas9
• Insert Size (bp): 4140
• Entrez Gene: NEWENTRY
• Promoter: Heterologous promoter from K. lactis (KLLA0F20031g)
• Tag / Fusion Protein:
  • SV40 NLS (C terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: KnpI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: .
• 3′ sequencing primer: .

Gene/Insert 2

• Gene/Insert name: KanMX marker expression cassette.
• Promoter: Heterologous TEF1 promoter from A. gossypii.

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: .
• 3′ sequencing primer: .

Resource Information

• Supplemental Documents:
  • pCSN061.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry