-
PurposeFor amplification of a miniAID-EGFP for C-terminal tagging
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 101713 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA5
- Total vector size (bp) 5912
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameminiAID-EGFP
-
Insert Size (bp)900
-
Tag
/ Fusion Protein
- miniAID-EGFP
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/182840v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA5/FRT miniAID-EGFP was a gift from Andrew Holland (Addgene plasmid # 101713 ; http://n2t.net/addgene:101713 ; RRID:Addgene_101713) -
For your References section:
Applying the auxin-inducible degradation system for rapid protein depletion in mammalian cells. Lambrus BG, Moyer TC, Holland AJ. Methods Cell Biol. 2018;144:107-135. doi: 10.1016/bs.mcb.2018.03.004. Epub 2018 Apr 5. 10.1016/bs.mcb.2018.03.004 PubMed 29804665