YPRCΔ15α2
(Plasmid
#101711)
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Purpose(Empty Backbone) GoldenBraid vector pDGB2α2, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101711 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepDGB2α1
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Backbone manufacturerGoldenBraid 2.0
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Modifications to backboneflanking of the GoldenBraid cloning cassette with YPRCΔ15 arms of homology for integration in the yeast genome
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
YPRCΔ15α2 was a gift from Elena Caro Bernat (Addgene plasmid # 101711 ; http://n2t.net/addgene:101711 ; RRID:Addgene_101711) -
For your References section:
Adaptation of the GoldenBraid modular cloning system and creation of a toolkit for the expression of heterologous proteins in yeast mitochondria. Perez-Gonzalez A, Kniewel R, Veldhuizen M, Verma HK, Navarro-Rodriguez M, Rubio LM, Caro E. BMC Biotechnol. 2017 Nov 13;17(1):80. doi: 10.1186/s12896-017-0393-y. 10.1186/s12896-017-0393-y PubMed 29132331
Included in kit:
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