pTriEX-Antenna-GDI Cdc42
(Plasmid #101681)

• Purpose: "Cdc42 was modified with a FRET ‘binding antenna’ that selectively reported Cdc42 binding to endogenous GDI..." -Hodgson et al
• Depositing Lab: Klaus Hahn
• Publication: Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/nchembio.2145.

Backbone

• Vector backbone: pTrieEX
• Backbone manufacturer: EMD Millipore
• Backbone size w/o insert (bp): 5238
• Total vector size (bp): 7038
• Vector type: Mammalian Expression, Bacterial Expression, Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cdc42
• Alt name: G25K GTP-binding protein
• Species: H. sapiens (human)
• Insert Size (bp): 2068
• GenBank ID: AAA52592.1
• Promoter: CMV
• Tags / Fusion Proteins:
  • 6xHis (N terminal on insert)
  • FRET Antenna (mCerulean-cpVenus) (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pTriEx up
• 3′ sequencing primer: pTriEx down

Resource Information

• Supplemental Documents:
  • Cdc42_GDI_antenna.gb
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry