pAB150
(Plasmid
#101661)
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PurposeOpto-T7RNAP*(563)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 101661 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSC101
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameOpto-T7RNAP*(563)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAB150 was a gift from Mustafa Khammash (Addgene plasmid # 101661 ; http://n2t.net/addgene:101661 ; RRID:Addgene_101661) -
For your References section:
Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control. Baumschlager A, Aoki SK, Khammash M. ACS Synth Biol. 2017 Nov 17;6(11):2157-2167. doi: 10.1021/acssynbio.7b00169. Epub 2017 Oct 18. 10.1021/acssynbio.7b00169 PubMed 29045151