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pCXN2-HA-AT1R-YFP
(Plasmid #101659)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101659 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCXN2
  • Total vector size (bp) 7831
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5alpha is also a suitable growth strain.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Angiotensin II type 1 receptor
  • Alt name
    AT1R
  • Alt name
    AGTR1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1114
  • GenBank ID
    NM_000685.4
  • Entrez Gene
    AGTR1 (a.k.a. AG2S, AGTR1B, AT1, AT1AR, AT1B, AT1BR, AT1R, AT2R1, ATR1, HAT1R)
  • Promoter CAG promoter
  • Tags / Fusion Proteins
    • HA (N terminal on insert)
    • YFP (Venus) (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (with HA) (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCXN2-HA-AT1R-YFP was a gift from Yusuke Ohba (Addgene plasmid # 101659 ; http://n2t.net/addgene:101659 ; RRID:Addgene_101659)

  • For your References section:

    Attenuation of ligand-induced activation of angiotensin II type 1 receptor signaling by the type 2 receptor via protein kinase C. Inuzuka T, Fujioka Y, Tsuda M, Fujioka M, Satoh AO, Horiuchi K, Nishide S, Nanbo A, Tanaka S, Ohba Y. Sci Rep. 2016 Feb 9;6:21613. doi: 10.1038/srep21613. 10.1038/srep21613 PubMed 26857745
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