Bongwoori-R3
(Plasmid
#101658)
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PurposeA variant of Bongwoori with H237R mutation in its linker region. (* super ecliptic pHluorin A227D)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101658 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBongwoori-R3
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Insert Size (bp)1443
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site Xho1 (not destroyed)
- 5′ sequencing primer CMV-forward
- 3′ sequencing primer BGH-R or custom (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Bongwoori-R3 was a gift from Bradley Baker (Addgene plasmid # 101658 ; http://n2t.net/addgene:101658 ; RRID:Addgene_101658) -
For your References section:
Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition. Lee S, Geiller T, Jung A, Nakajima R, Song YK, Baker BJ. Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.1038/s41598-017-08731-2. 10.1038/s41598-017-08731-2 [pii] PubMed 28811673
