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Addgene

CC1-M240
(Plasmid #101657)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101657 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CC1-M240
  • Insert Size (bp)
    1446

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Nhe1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer CMV-forward
  • 3′ sequencing primer BGH-R or custom
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CC1-M240 was a gift from Bradley Baker (Addgene plasmid # 101657 ; http://n2t.net/addgene:101657 ; RRID:Addgene_101657)

  • For your References section:

    Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition. Lee S, Geiller T, Jung A, Nakajima R, Song YK, Baker BJ. Sci Rep. 2017 Aug 15;7(1):8286. doi: 10.1038/s41598-017-08731-2. 10.1038/s41598-017-08731-2 [pii] PubMed 28811673
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