pGS_N_3xFLAG
(Plasmid
#101654)
-
PurposeExpression of type I signal-peptide containing proteins with N-terminal 3xFLAG-tag in insect cells
-
Depositing Labs
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101654 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepACEBac1
-
Backbone manufacturerGeneva Biotech
- Total vector size (bp) 3004
-
Vector typeInsect Expression
Growth in Bacteria
-
Bacterial Resistance(s)Gentamicin, 10 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameLRP6 Signal peptide, KpnI linker, 3xFLAG tag
-
SpeciesSynthetic
-
Insert Size (bp)186
-
Tag
/ Fusion Protein
- 3xFLAG tag (N terminal on backbone)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pGS_N_3xFLAG was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 101654 ; http://n2t.net/addgene:101654 ; RRID:Addgene_101654) -
For your References section:
Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain. Le LTM, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. 10.1007/s12033-017-0041-8 [pii] PubMed 29143175
