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Addgene

pGS_N_Strep
(Plasmid #101651)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101651 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pACEBac1
  • Backbone manufacturer
    Geneva Biotech
  • Total vector size (bp) 3022
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LRP6 Signal peptide, KpnI linker, Twin-Strep tag
  • Species
    Synthetic
  • Insert Size (bp)
    204
  • Tag / Fusion Protein
    • Twin-Strep tag (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS_N_Strep was a gift from Monika Golas & Bjoern Sander (Addgene plasmid # 101651 ; http://n2t.net/addgene:101651 ; RRID:Addgene_101651)

  • For your References section:

    Vectors for Expression of Signal Peptide-Dependent Proteins in Baculovirus/Insect Cell Systems and Their Application to Expression and Purification of the High-Affinity Immunoglobulin Gamma Fc Receptor I in Complex with Its Gamma Chain. Le LTM, Nyengaard JR, Golas MM, Sander B. Mol Biotechnol. 2018 Jan;60(1):31-40. doi: 10.1007/s12033-017-0041-8. 10.1007/s12033-017-0041-8 [pii] PubMed 29143175
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