pRSM14
(Plasmid #101645)

• Purpose: Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.
• Depositing Lab: Thomas Hughes
• Publication: Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25.

Backbone

• Vector backbone: pNCS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: efasCFP
• Insert Size (bp): 684
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The fluorescent protein sequence comes from the following publication: Alieva N. O.; Konzen K. A.; Field S. F.; Meleshkevitch E. A.; Hunt M. E.; Beltran-Ramirez V.; Miller D. J.; Wiedenmann J.; Salih A.; Matz M. V. Diversity and Evolution of Coral Fluorescent Proteins. PLoS One 2008, 3, e2680.10.1371/journal.pone.0002680.