pRSM4
(Plasmid #101642)

• Purpose: Encodes the fluorescent protein KCyG4219 for constitutive expression in E. coli.
• Depositing Lab: Thomas Hughes
• Publication: Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25.

Backbone

• Vector backbone: pNCS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KCyG4219
• Insert Size (bp): 660
• Mutation: C-terminal residues (221-223) deletion. Ser220Val, Lys11Arg, Phe13Tyr, Lys32Arg, Gln187Lys, Ala131Met, Asn200Glu, Cys67Ala, Gly205Lys. Insertion of Val between Met1 and Ser2.
• Tag / Fusion Protein:
  • 6XHis (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The fluorescent protein sequence comes from the following publication: Kikuchi A.; Fukumura E.; Karasawa S.; Shiro Y.; Miyawaki A. Crystal Structure of a New Cyan Fluorescent Protein and Its Hue-Shifted Variants. Biochemistry 2009, 48, 5276_528310.1021/bi801658p.