pRSM17
(Plasmid
#101641)
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PurposeEncodes the fluorescent protein eqFP486 for constitutive expression in E. coli.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 101641 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNCS
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeqFP486
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Insert Size (bp)687
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Tag
/ Fusion Protein
- 6XHis (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The fluorescent protein sequence was found at GenBank Accession AF545829.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSM17 was a gift from Thomas Hughes (Addgene plasmid # 101641 ; http://n2t.net/addgene:101641 ; RRID:Addgene_101641) -
For your References section:
Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, Hughes TE, Drobizhev M. J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25. 10.1021/acs.jpclett.7b00960 PubMed 28530831
