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Addgene

pThom77.6
(Plasmid #101637)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101637 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUB2.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    amFP486/K68M
  • Insert Size (bp)
    684
  • Mutation
    K68M
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The wild type fluorescent protein sequence comes from the following publication: Matz M. V.; Fradkov A. F.; Labas Y. A.; Savitsky A. P.; Zaraisky A. G.; Markelov M. L.; Lukyanov S. A. Fluorescent Proteins from Nonbioluminescent Anthozoa Species. Nat. Biotechnol. 1999, 17, 969_97310.1038/13657.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pThom77.6 was a gift from Thomas Hughes (Addgene plasmid # 101637 ; http://n2t.net/addgene:101637 ; RRID:Addgene_101637)

  • For your References section:

    Blue-Shifted Green Fluorescent Protein Homologues Are Brighter than Enhanced Green Fluorescent Protein under Two-Photon Excitation. Molina RS, Tran TM, Campbell RE, Lambert GG, Salih A, Shaner NC, Hughes TE, Drobizhev M. J Phys Chem Lett. 2017 Jun 15;8(12):2548-2554. doi: 10.1021/acs.jpclett.7b00960. Epub 2017 May 25. 10.1021/acs.jpclett.7b00960 PubMed 28530831
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