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Addgene

pDONR221-HES5
(Plasmid #101409)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101409 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR221
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Gateway shuttling vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HES5
  • Alt name
    Q5TA89 (Uniprot ID)
  • Species
    H. sapiens (human)
  • Mutation
    Last nucleotide of stop codon removed to allow for Gateway C-terminal fusions
  • Entrez Gene
    HES5 (a.k.a. bHLHb38)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Harvard generated by gene synthesis

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDONR221-HES5 was a gift from George Church (Addgene plasmid # 101409 ; http://n2t.net/addgene:101409 ; RRID:Addgene_101409)

  • For your References section:

    A comprehensive library of human transcription factors for cell fate engineering. Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal M, Melero-Martin JM, Busskamp V, Church GM. Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 10.1038/s41587-020-0742-6. Epub 2020 Nov 30. 10.1038/s41587-020-0742-6 PubMed 33257861
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