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Addgene

pDD2031
(Plasmid #101374)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101374 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II (KS+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 7150
  • Vector type
    Unspecified

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
  • Species
    KSHV (viral)
  • Mutation
    RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene's sequencing found V466A and R510C mutations in CBG68luc and a V298A mutation in CBRluc. These mutations are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDD2031 was a gift from Dirk Dittmer (Addgene plasmid # 101374 ; http://n2t.net/addgene:101374 ; RRID:Addgene_101374)

  • For your References section:

    Quantitative analysis of the bidirectional viral G-protein-coupled receptor and lytic latency-associated nuclear antigen promoter of Kaposi's sarcoma-associated herpesvirus. Hilton IB, Dittmer DP. J Virol. 2012 Sep;86(18):9683-95. doi: 10.1128/JVI.00881-12. Epub 2012 Jun 27. 10.1128/JVI.00881-12 PubMed 22740392
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