pNL4-3 envFS eGFP NoStarts
(Plasmid #101342)

• Purpose: All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG
• Depositing Lab: Jeremy Luban
• Publication: McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi: 10.1038/s41467-018-07753-2.

Backbone

• Vector backbone: pNL4-4
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NL4-3
• Mutation: No methonines in Gag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The last methionine in Gag (M423) was left as it was outside the ApaI site used to clone in the mutations. However, C-terminus of Gag is functionally inert, and Pol slips 27 nucleotides away and is unlikely to produce a stable protein product.