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Addgene

pMflET-o4
(Plasmid #101314)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    colE1
  • Vector type
    Bacterial Expression, Synthetic Biology
  • Selectable markers
    Erythromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
  • Alt name
    Complete oriC region of M. florum L1
  • Species
    Mesoplasma florum

Gene/Insert 2

  • Gene/Insert name
    ereB resistance gene

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMflET-o4 was a gift from Sébastien Rodrigue (Addgene plasmid # 101314 ; http://n2t.net/addgene:101314 ; RRID:Addgene_101314)

  • For your References section:

    Development of oriC-Based Plasmids for Mesoplasma florum. Matteau D, Pepin ME, Baby V, Gauthier S, Arango Giraldo M, Knight TF, Rodrigue S. Appl Environ Microbiol. 2017 Mar 17;83(7). pii: e03374-16. doi: 10.1128/AEM.03374-16. Print 2017 Apr 1. 10.1128/AEM.03374-16 PubMed 28115382
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