aCat DeltaTS (delta570 to 670 aa in aCat TS WT)
(Plasmid #101300)

• Purpose: FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.
• Depositing Lab: Alpha Yap
• Publication: Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867. doi: 10.1016/j.celrep.2017.02.078.

Backbone

• Vector backbone: pEGFP-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: alpha-Catenin
• Species: M. musculus (mouse)
• Mutation: Contains amino acids 1-509, 670-698, and 699-906 of alpha-Catenin

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • Annotated Sequence
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For alpha-CatD-TS, the alpha-Cat-TS was digested with NheI and XmaI to cut out a fragment corresponding to 1-670 aa, and a PCR-amplified fragment corresponding to amino acids 1_509 from mouse aE-catenin was ligated back at the same restriction enzyme sites