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Addgene

MRLC-DD-pIRES-EGFP-AP4-mito
(Plasmid #101287)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 101287 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1(-)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MRLC-DD and AP4-Mito
  • Species
    Synthetic
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

MRLC-DD: Myosin regulatory light-chain transgene and AP4-Mito:a transgene bearing ActA AP4 motifs fused to a mitochondrial targeting sequence (INACTIVE)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MRLC-DD-pIRES-EGFP-AP4-mito was a gift from Alpha Yap (Addgene plasmid # 101287 ; http://n2t.net/addgene:101287 ; RRID:Addgene_101287)

  • For your References section:

    Tension-sensitive actin assembly supports contractility at the epithelial zonula adherens. Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman BD, Grashoff C, Schwartz MA, Yap AS. Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 10.1016/j.cub.2014.06.028. Epub 2014 Jul 24. 10.1016/j.cub.2014.06.028 PubMed 25065757
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